Simultaneous Determination of 4 Flavonoids in Inula britannica by RP-HPLC / 中国药房

ABSTRACT

OBJECTIVE:To establish the RP-HPLC method for simultaneous determination of flavonoids(quercetin,cyanidenon,spinacetin,and 6-methoxyl-cyanidenon) in Inula britannica. METHODS:The samples were separated on chromatographic column Inertsil ODS-3(250 mm?4.6 mm,5 ?m) at a column temperature of 40 ℃.The mobile phase consisted of methanol-acetonitrile-0.49% citric acid(gradient elution) at a flow rate of 1.0 min?mL-1. The detection wavelength was set at 360 nm. RESULTS:The linear ranges of quercetin,cyanidenon,spinacetin,and 6-methoxyl-cyanidenon were 0.17～6.8 ?g(r=0.999 9),0.088～3.52 ?g(r=0.999 9),0.020 2～0.808 ?g(r=0.999 9) and 0.045～1.8 ?g(r=0.999 6),respectively,and the average recoveries of all stood at 96.03%～99.78% and RSD at 0.73%～1.36%(n=6 each). CONCLUSION:The method is simple,sensitive,accurate and reproducible,and it is applicable for the quality control of the raw medicinal substance and preparation of I.britannica.