Determination of the Entrapment Efficiency of Tetracaine Hydrochloride Liposome by the Microcolumn Centrifugation-HPLC / 中国药房
China Pharmacy
;
(12)2007.
Article
in Chinese
| WPRIM
| ID: wpr-532946
ABSTRACT
OBJECTIVE:
To develop a method for determining the entrapment efficiency of tetracaine hydrochloride liposome.METHODS:
The microcolumn centrifugation was adopted to separate the free tetracaine hydrochloride from liposomes;the content of tetracaine hydrochloride was determined by HPLC and the entrapment efficiency was computed.RESULTS:
By microcolumn centrifugation,the liposomes and the free tetracaine hydrochloride were well-separated.The recovery rate of the blank liposomes was 90.4%~100.1%;the absorption rate of free tetracaine hydrochloride was 96.6%~99.2%;and the entrapment efficiencies of tetracaine liposomes stood at about 80%.CONCLUSION:
This method established for determining the entrapment efficiency of tetracaine hydrochloride liposome is simple,rapid and reproducible.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2007
Type:
Article
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