Inhibitory effect of ground dragon on the expression of ?-SMA and FN in the lung tissue of mouse with asthma / 中国病理生理杂志

ABSTRACT

AIM:To investigate the inhibitory effect of ground dragon on the expression of ?-SMA and FN in the lung tissue with asthma.METHODS:The BALB/c mice were divided into four groupscontrol group(group A,n=20),asthmatic model group(group B,n=20),large-dose ground dragon treatment group(group C,n=20)and low-dose ground dragon treatment group(group D,n=20).To establish a mouse model of chronic asthma,we sensitized the mouse with 0.02% ovalbumin(OVA)by intraperitoneal injection,and stimulated the mice with 1% OVA by atomization.The treatment groups were given ground dragon before stimulation every time.After the last time of stimulation,the mice were subjected to laboratory tests.Inflammatory cells in bronchoalveolar lavage fluid were counted.Total level of IgE in serum was detected by ELISA.FN mRNA and ?-SMA mRNA in the lung tissue were measured by RT-PCR and AlphaImager 2200 semi-quantitation analysis system.Expressions of FN and ?-SMA were measured by the method of two-step immunohistochemistry and leica QWIN V3 analysis system.RESULTS:(1)Compared with those in group A,the expressions of ?-SMA and FN in group B were significantly increased(P0.05).(2)Compared with those in group A,the expression levels of ?-SMA mRNA and FN mRNA in group B had a great increase(P0.05).CONCLUSION:The ground dragon inhibits ?-SMA and FN expression in the lung tissue of mice with chronic asthma,indicating that ground dragon may inhibit airway remodeling in asthma through the inhibition of ?-SMA and FN expressions.