Determination of Theophylline Concentration in Human Plasma by Micellar Electrokinetic Capillary Chromatography / 中国药房
China Pharmacy
;
(12)2007.
Article
in Chinese
| WPRIM
| ID: wpr-532998
ABSTRACT
OBJECTIVE:
To develop a simple micellar electrokinetic capillary chromatography for determination of theophylline concentration in human plasma.METHODS:
The determination of plasma sample(without any pretreatment) was performed on un-coated fused-silica capillaries (at an effective length of 21 cm) in which the cathodic buffer solution (solution A) was 15 mmol?L-1 borax(pH=10) and the running buffer solution(solution B) was sodium dodecyl sufate (SDS)(200 mmol?L-1) -contained solution A. The sample was injected into capillary by pressure with separation voltage of 12 kV;the UV detection wavelength was set at 280 nm;quantitation was performed by external standard peak area method.RESULTS:
The average determination time of each sample was 11 min;the linear range of theophylline was 1.25~40 ?g?mL-1 with its lowest detectable limit at 0.5 ?g?mL-1;the average methodological recovery was 90.33%(RSD=2.51%);the intra-day RSD was 2.34%~3.36% and the inter-day RSD was 2.03%~6.51%,respectively.CONCLUSION:
The developed method is simple,rapid and sensitive and it is applicable for the therapeutic drug monitoring of theophylline.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2007
Type:
Article
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