Extraction of DNA from Corydalis saxicola:Comparison among Different Extraction Methods / 中国药房

ABSTRACT

OBJECTIVE:To draw a comparison among four extraction methods of total DNA from Corydalis saxicola in order to get high quality DNA sample.METHODS:The total DNA was extracted by palmityl trimethyl ammonium bromide method,enzyme-added detergent method,guanidinium isothiocyanate method,inertmacromolecular adsorptive column method,respectively.The purity(defined as RA260/A280)and the yield of the total DNA were determined.RESULTS:The RA260/A280 values of the DNA samples extracted by 4 methods were 1.832 9,1.699 2,1.812 2 and 1.813 6,respectively;and the extraction rates were 140.20 ?g?g-1,69.56 ?g?g-1,132.50 ?g?g-1 and 143.12 ?g?g-1,respectively.CONCLUSION:DNA samples extracted in 4 methods were all of high quality.The yield of DNA sample in enzyme-added extraction method was lower,while the guanidinium isothiocyanate method and the adsorption column method were rapid and simple and they are suitable for the test of samples of small size.