Assay of in Vitro Plasma Protein Binding Rate of Mangiferin in Rats / 中国药房

ABSTRACT

OBJECTIVE:To study the assay method of in vitro plasma protein binding rate of mangiferin in rats.METHODS:The plasma samples which were added with internal standard(rutin)were deproteinized with acetonitrile and the supernatant was assayed by HPLC.The equilibrium dialysis was carried out to determine the plasma protein binding rate of mangiferin.RESULTS:The plasma protein binding rates of mangiferin at low,middle and high concentrations were(50.0?1.9)%,(45.2?2.0)% and(45.9?2.1)%,respectively.CONCLUSION:This assay method is sensitive,reproducible and simple,and it meets the requirement of analysis.