Determination of the in Vitro Release Rate of Gatifloxacin Carried by Medical Biofibrin Glue / 中国药房

ABSTRACT

OBJECTIVE:To establish a method for determination of the in vitro release rate of gatifloxacin carried by medical biofibrin glue (MBG-Gat).METHODS:The content of Gat was determined by HPLC on ZORBAX300SB-C18 column with column temperate kept at 30 ℃ and detection wavelength was 293 nm;the mobile phase consisted of potassium dihydrogen phosphate buffered solution (pH 3.5)-acetonitrile (76∶24) at a flow rate of 1 mL?min-1.The volume of injection was 20 ?L.MBG-Gat was placed in normal saline for drug release,with the release solution determined in every 12 h for consecutive 9 days.RESULTS:The linear range of Gat was 5～80 ?g?mL-1 (r=0.999 7) with an average recovery rate of 99.77%～103.53%,RSD≤2.77%(n=5).The accumulative release rate of MBG-Gat in normal saline within 9 days reach-ed above 70%.CONCLUSION:The established method for determination of the in vitro drug release of Gat is proved to be sensitive and reproducible,and it is applicable for the determination and analysis of Gat in vitro.The Gat carried by MBG is characterized by sustained release.