Determination of Theophylline in Pulsatile Controlled-release Pellets Capsule of Theophylline by HPLC / 中国药房

ABSTRACT

OBJECTIVE:To establish a HPLC method for the determination of theophylline in pulsatile controlled-release pellets capsule of theophylline. METHODS: The determination was performed on a Diamonsil C18 column with the mobile phase consisted of methanol -water (35∶65) at a flow rate of 1.0 mL?min-1. The detection wavelength was set at 272 nm and the injection volume was 20 ?L. RESULTS: The linear range of theophylline was 5～40 ?g?mL -1 (r=0.999 9) with an average recovery rate of 100.55%. Both the intra-day RSD and the inter-day RSD were less than 1.2%. CONCLUSION: The method is accurate,reliable,simple and feasible,and it is applicable for the content determination of this preparation.