Determination of Gentiopicroside in Gentian Liver-purging Pill by HPLC / 中国药房

ABSTRACT

OBJECTIVE:To establish an HPLC method for determination of gentiopicroside in Gentian liver-purging pill. METHODS:The chromatographic separation was carried out on VP-ODS(150 mm?4.6 mm,5 ?m). The mobile phase consisted of acetonitrile-0.1% phosphoric acid solution(15∶85) at a flow rate of 1.0 mL?min-1.The UV detection wavele-ngth was set at 270 nm and column temperature was kept at 25 ℃.RESULTS:The liner range of gentiopicroside was 0.355 4～5.331 ?g(r=0.999 8) and the average recovery was 100.05%(RSD=2.2%,n=6). CONCLUSION:The method is simple,rapid and accurate,and it is applicable for the quality control of Gentian liver-purging pill.