Optimization of Cellulase-assited Extraction Technology of the Steam-processed Scutellaria baicalensis by Orthogonal Experiment / 中国药房

ABSTRACT

OBJECTVIETo optimize the cellulase-assisted extraction technology of steam-processed Scutellaria baicalensis. METHODS:The cellulase-assisted extraction technology of steam-processed S.baicalensis was optimized by orthogonal experiment with the extraction rates of baicalin and wogonoside as indexes and with cellulase concentration,extracting temperature,extracting time and pH value as factors. RESLUTSThe optimal cellulase-assisted extraction technology of steam-processed S.baicalensis was as followsthe concentration of cellulase was 15 U?g-1; the extracting temperature was 50 ℃; the extracting time was 7 h and the pH value was 4.8. Under the optimal conditions,the extraction rates of baicalin and wogonoside were 8.44% and 2.62%,respectively. CONCLUSION:The cellulase-assisted extraction technology of steam-processed S.baicalensis is superior to the traditional water-decoction method,and it proves to be an effective process for the extraction of baicalin and wogonoside from steam-processed S.baicalensis and by which the utilization rate of S.baicalensis can be improved.