Determination of AtractylenolideⅠ and Atractylone in Chang’ankang Granule by HPLC / 中国药房

ABSTRACT

OBJECTIVE: To develop a method for determination of atractylenolide Ⅰ and atractylone in Chang’ankang granule by HPLC. METHODS: The determination was performed on Yilite ODS column (250 mm?4.6 mm,5 ?m). The mobile phase contained of acetonitrile-water (gradient elution) with flow rate of 1 mL?min-1 and detection wavelength of 220 nm. RESULTS: The linear range of atractylenolide Ⅰwere 0.1～2.0 ?g(r=0.999 9) with mean recovery of 98.84%(RSD=0.76%,n=6).The linear range of atractylone were 0.5～10.0 ?g with mean recovery of 98.43%(RSD=1.14%,n=6). CONCLUSION: This method is simple, convenience and accurate for quality control of Chang’ankang granule.