JNK mediates TNF-? or H_2O_2-induced insulin resistance in 3T3-L1 adipocytes / 中国病理生理杂志

ABSTRACT

AIM:To study the role of c-Jun NH2-terminal kinase (JNK) in the development of insulin resistance induced by tumor necrosis factor-? (TNF-?) or H2O2 in 3T3-L1 adipocytes. METHODS:Differentiated 3T3-L1 adipocytes were pretreated with JNK1 small interfering RNA (siRNA) or JNK inhibitor SP600125,then exposed to 1 nmol/L of TNF-? or micromolar H2O2 generated by adding glucose oxidase (50 U/L) to the medium for 12 h. The cellular glucose uptake was determined by radioactive method. RESULTS:Compared to control adipocytes,12 h incubation with TNF-? or H2O2 led to 50%-55% reduction (P