Content Determination of Polysaccharide in Different Compatibility of Fengshao Liujunzi Decoction / 中国药房

ABSTRACT

OBJECTIVE: To determine polysaccharide in different compatibility of Fengshao liujunzi decoction.METHODS: Polysaccharide was extracted with water extracting-alcohol precipitation.Polysaccharide was prepared by anthrone-sulfuric acid method and determined by UV spectrophotometry.RESULTS: The optimal determination conditions for polysaccharide in Fengshao liujunzi decoction were as follows: 4 mL 0.2% anthrone-sulfuic acid solution was added into sample,heated in water bath for 15 min then cooled for 10 min.The linear range of D-glucosum anhydricum were 0.019 92～0.079 68 mg(r=0.999 6).The average recovery was 97.33%(RSD=1.52%,n=6).CONCLUSION: Polysaccharide is mainly from Liujunzi decoction.The method is rapid,accurate and reproducible for content determination of polysaccharide in different compatibility of Fengshao liujunzi decoction.