Simultaneous Determination of Anethole Trithione and Its Metabolite in Plasma of Beagle Dogs by HPLC / 中国药房
China Pharmacy
;
(12)2001.
Article
in Chinese
| WPRIM
| ID: wpr-533616
ABSTRACT
OBJECTIVE:
To establish an HPLC method for the determination of anethole trithione(AT)and its metabolite 4-hydroxy-anethole trithione(ATX)in plasma of beagle dogs simultaneously.METHODS:
Protein in plasma was deposited directly using acetonitrile and analyzed by supernate. The separation was performed on Diamonsil ODS-C18 column with a mobile phase canodn sAisTtiXng woefr em ectohmanpolel tealnyd swepaaterar(ted8 0w∶i2t0h)ouat t ian teflrofewr ernactee . oTf h1e. 0r emteLn?timoni nt-im1 we iwtha sth 5e. 5d emteicnt iaonnd w 3a.v4e mleinng trhe sopfe c3t4iv6e nlym. .T RheE SliUneLaTr SranAgTe were 0.254~3.392 ?g?mL-1(r=0.999 9)for AT with average recovery of(93.89?2.39)% and 0.125~1.664 ?g?mL-1 for ATX (r=0.999 8)with average recovery of(96.28?2.83)%. The intra-day RSD and inter-day RSD of AT were 0.83%~4.11% and 0.71%~4.51% while that of ATX were 0.55%~1.23% and 1.74%~3.92%,respectively. The detection limit of AT in human plasma was 0.254 ?g?mL-1 and that of ATX was 0.125 ?g?mL-1.CONCLUSION:
It is proved that established method is simple,accurate and reproducible for plasma concentration determination of AT and ATX in plasma of beagle dogs.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2001
Type:
Article
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