Effect of Meloxicam on nasopharygeal carcinoma CNE-1 cell line / 中国耳鼻咽喉头颈外科

ABSTRACT

OBJECTIVE To study the effect of Meloxicam on the growth inhibition and apoptosis induction in nasopharyngeal carcinoma CNE-1 cell line.METHODS Technique of cell culture and randomized blank-contrast design were used.The degree and dose-dependency of Meloxicam which induced growth inhibition and apoptosis in CNE-1 cell line were observed by MTT method,AO+EB straining and flow cytomethy.COX-2 were determined using cell immunoflurorescence.RESULTS MTT assay showed that Meloxicam inhibite the growth of CNE-1 cells.AO+EB straining showed partial cells presented characteristic morphological changes of apoptosis.Flow cytometry analysis showed that treating CNE-1 cells with Meloxicam increase the percentage of apoptosis cells.Cell immunoflurorescence revealed that the COX-2 expression was decreased.CONCLUSION Meloxicam could significantly inhibit the growth and induce apoptosis of CNE-1 cells.Apoptosis of tumor cells is closely associated with down regulation of the ratio of COX-2.