Investigation of KGF and KGFR expression in external auditory canal skin adjacent to tympanic membrane perforation / 中国耳鼻咽喉头颈外科

ABSTRACT

OBJECTIVE To investigate the continuous expression of keratinocyte growth factor(KGF)and keratinocyte growth factor receptor(KGFR)in external auditory canal skin adjacent to tympanic membrane perforation and analyze the role of KGF and KGFR in the different turnover of chronic otitis media.METHODS The external auditory canal skin adjacent to tympanic membrane perforation from 20 cases with cholesteatoma otitis media and the corresponding cholesteatoma tissue and normal external ear skin were examined by immunohistochemical S-P method and quantitative analysis.The positive rate was compared with 20 cases of non-cholesteatom otitis media's external auditory canal skin adjacent to tympanic membrane perforation.RESULTS The staining for KGF and KGFR in cholesteatoma otitis media's external auditory canal skin adjacent to tympanic membrane perforation was consistently stronger than that in non-cholesteatoma otitis media.The positive rates of the two tissue was(33.135?6.364)% and(19.965?10.570)%,(19.380?2.827)% and(13.145?7.935)% respectively,revealing a significant difference.CONCLUSION The activity of hyperproliferation of the external auditory canal skin adjacent to tympanic membrane perforation in cholesteatoma otitis media is stronger than that of non-cholesteatoma otitis media.KGF and KGFR may play a more important role for hyperproliferation of cholesteatoma.