Determination of Vancomycin in Human Serum by HPLC / 中国药房
China Pharmacy
;
(12)2005.
Article
in Chinese
| WPRIM
| ID: wpr-533970
ABSTRACT
OBJECTIVE:
To establish an HPLC method for the determination of the concentration of vancomycin in human serum.METHODS:
Serum samples were centrifuged after serum protein was precipitated by 20% metaphosphoric acid. The separation was performed on Hypersil C18 column with mobile phase consisted of acetonitrile-0.012 5 mol?L-1 potassium dihydrogen phosphate buffer (10 ∶ 90) at flow rate of 0.8 mL?min-1. The detection wavelength was set at 236 nm and column temperature was set at 25 ℃.RESULTS:
The average relative recovery rate of vancomycin in low, medium and high concentrations (2.5,30.8,150.4 mg?L-1) were 99.8%, 101.1% and 98.9% respectively. The inter-day and intra-day RSD were less than 2.5%(n=5). The limit detection of vancomycin in serum was 2.0 mg?L-1. The linear range was within 2.0~170.0 mg?L-1(r=0.999 8).CONCLUSION:
The method is simple, accurate, and rapid for the monitoring and phamacokinetic studies of vancomycin in human serum.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2005
Type:
Article
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