Down-regulation of GEP100 inhibits the invasive ability of pancreatic cancer cell AsPC-1 in vitro / 中国病理生理杂志

ABSTRACT

AIM:To study the effects of down-regulation of guanine nucleotide exchange protein 100 (GEP100) on the invasive ability of pancreatic cancer cell AsPC-1 in vitro.METHODS:The clone of AsPC-1 cells with stable knock-down of GEP100 by transfection of pSuper-retro-puro-GEP100 was established.The invasive ability was evaluated by matrigel invasion assay and the migratory ability of the cells was examined by crossing-river test.The protein expression of E-cadherin was determined by Western blotting.RESULTS:The invasive ability was inhibited significantly in matrigel invasion assay (P