Content Determination of Berberine in Fujie Lotion by RP-HPLC / 中国药房
China Pharmacy
;
(12)2007.
Article
in Chinese
| WPRIM
| ID: wpr-534360
ABSTRACT
OBJECTIVE:
To develop an RP-HPLC method for the content determination of berberine in Fujie lotion.METHODS:
The separation was performed on Agilent 1200 technologies XDB-C18(250 mm?4.6 mm,5?m)column with acetonitrile-0.02%monobasic potassium phosphate(4555)as mobile phase at flow rate of 1.0 mL?min-1.The UV detection wavelength was set at 265nm.RESULTS:
The linear range of berberine were 0.029 6~0.236 8?g(r=0.999 1)with an average recovery of 99.21%(RSD=1.10%,n=9).CONCLUSION:
Established method is sensitive,simple,rapid,accurate and reproducible for the quality control of Fujie lotion.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2007
Type:
Article
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