Construction and identificication of siRNA recombinant expression vector targeting HIF-1? gene / 中国普通外科杂志

ABSTRACT

Objective To construct siRNA recombinant expression vector targeting HIF-1? gene.Methods Genome sequences of HIF-1? gene were retrieved from gene bank,and four pairs of oligonucleotides were synthesized and inserted into plasmid pGensil-1.Then,DH5? strains were transformed,and plasma was extracted and identified by restriction endonuclease and sequence analysis.Results It was confirmed by sequence analysis that siRNA recombinant expression vector targeting HIF-1? gene coincided completely with the designs.Conclusions The siRNA recombinant expression vector targeting HIF-1? gene has been constructed successfully and lays a foundation for furher study on the biological behavior of tumors.