Content Determination of Chlorogenic Acid in Different Dosage Forms of Qingkailing Preparation by RP-HPLC / 中国药房
China Pharmacy
;
(12)2007.
Article
in Chinese
| WPRIM
| ID: wpr-534501
ABSTRACT
OBJECTIVE:
To establish RP-HPLC method for the content determination of chlorogenic acid in 3 kinds of dosage forms of Qingkailing preparation.METHODS:
SinoChrom ODS-AP C18(250 mm?4.6 mm, 5 ?m) column was adopted and the mobile phase consisted of methnol-water-acetic acid (25 ∶ 75 ∶ 0.5) at the flow rate of 1.0 mL?min-1. Column temperature was set at 30 ℃ and the detection wavelength was set at 327 nm.RESULTS:
The linear range of chlorogenic acid was 3.81~152.50 ?g?mL-1(r=0.999 8), and the average recoveries of the capsules, granules and dripping pills were 99.03%(RSD=2.02%), 99.35%(RSD=1.16%),98.11%(RSD=1.51%).CONCLUSION:
The method is sensitive, simple and accurate for the quality control and content determination of 3 kinds of Qingkailing preparations.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2007
Type:
Article
Similar
MEDLINE
...
LILACS
LIS