Content Determination of Astragaloside Ⅳ in Qizhi Jiangtang Granules by HPLC-ELSD / 中国药房
China Pharmacy
;
(12)2007.
Article
in Chinese
| WPRIM
| ID: wpr-534534
ABSTRACT
OBJECTIVE:
To establish HPLC-ELSD method for content determination of astragaloside Ⅳ in Qizhi jiangtang granules.METHODS:
The separation was performed on Hypersil ODS2(250 mm?4.6 mm,5 ?m)column with acetonitrile-water(32 ∶ 68)as mobile phase. The flow rate was 1.0 mL?min-1. Injection volume was 10 ?L. The column temperature was 25 ℃. The temperature of drift tube for measurement was 110 ℃ and flow rate of N2 was 3.12 SLPM.RESULTS:
Regression equation was A=1.699 C+6.181. The linear range of astragaloside Ⅳ was 0.259~1.295 mg?mL-1(r=0.999 6,n=5) with lowest detectable limit of 5.18 ?g?mL-1. An average recovery rate were 97.75%(RSD=2.17%).CONCLUSION:
The method is simple,rapid and reliable for quality control of Qizhi jiangtang granules.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2007
Type:
Article
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