The Production and Characterization of Monoclonal Antibody to Human Chorionic Gonadotropin(hCG) / 中国免疫学杂志

ABSTRACT

Splenocytes from BALB/c mice immunized with human chorionic gonadotropin(hCG) were fused with myeloma cells NS-1, and 7 hybridomas producing monoclonal antibodies to hCG were established. By using RIA to measure the binding characteristics of these monoclonal antibodies to ~(125)I-labelled hCG, their subunits, and 4 glycoprotein pitiutary hormones, 7 antibodies had been devided into 3 groups. Of these groups one(4 antibodies included) recognized intact hCG and ?-subunit, a second(one antibody only) recognized intact hCG and ?-subunit, and the third(2 antibodies included) bound to the hCG molecule only, The precipitation after double diffusion in agar with hCG antigen demonstrated that 4 antibodies of group 1 recognized 3 epitopes, and 2 antibodies of group 3 recognized different epitopes. The cooperation between pairs of antibodies specific to different epitopes in binding hCG antigen showed that most of these antibodies cooperated each other. The antibody No 10 was showed to be the best, cooperating with every other antibody,where as antibody No 22 was cooperated only with a few antibodies.The coorperation was related to the differency of epitopes recognized, but seemed not to bear relationship with the titre of antibodies.