Ⅴ.Studies of human lymphocyte surface antigens Antigenic determinants recognized by monoclonal antibodies anti-CCT1 and anti-CCT3 and their function / 中国免疫学杂志

ABSTRACT

We have previously described two monoclonal antibodies,anti-CCT1 and antiCCT3,which recognize TP50 protein(associated with E-receptor)on human T cell surface.In this paper,the results of further studies on the determinants the two monolonal antibodies recognize and their functions are presented.anti-CCT3 blocked E rosette formation but anti-CCT1 did not.However,CCT1 and CCT3 determinantscould be.co-modulated by both monoclonal antibodies.When T cells were treated withboth antibodies simultaneously,the indirect immunofluorescence revealed by cytofluo-rograf was additive.Functionally,anti-CCT1 and anti-CCT3 were similar.Bothinhibited the proliferative response of peripheral blood lynphocyte to PHA and theexpression of IL-2 receptor.These results indicate that CCT1 and CCT3 are twodifferent epitopes on the TP50 molecules and are functionelly involved in the pro-liferation of T cells in response to mitogens.