A BA-ELISA-Spot assay for the detection and enumeration of speifc antibody seretng cells / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)1985.
Article
in Chinese
| WPRIM
| ID: wpr-534719
ABSTRACT
A new solid-phase BA-ELISA-spot assay is described for detection and enumera-tion of antigen specific antibody secreting cells.Spleen cells from immunized mice areincubated in plstic wells precoated with specific an tigen.After removal of the cells,immobilized antibodies at the point of release from single cell are demonstrated bymeans of an immunenzyme procedure in which.enzyme-substrate(HRP-TMB)reactionsare performed.Blue spots are thus produced and counted with naked eye.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
1985
Type:
Article
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