PREPARATION OF MONOCLONAL ANTIBODIES AGAINST HUMAN VASCULAR ENDOTHELIAL CELLS AND THEIR POSSIBLE APPLICATION IN VASCULAR ENDOTHELIAL CELL TYPING / 中国免疫学杂志

ABSTRACT

Human vascular endothelial cells(VEC)were isolated from umbilicalyeins by trypsin digestion.Spleen cells from BALB/c mice having been im-munized with the isolated endothelial cells were fused with the mousemyeloma cell line SP2/0.After screening and subcloning,three differentmonoclonal antibodies(EC1,EC2,EC3)against human umbilical VEC wereobtained.These antibodies were proved to belong to immunoglobulin sub-classes(IgG_2,IgG_3,IgG_2).EC1 was shown to bind strongly to the vascularendothelial cell membrane,but not to react with peripheral lymphocytes andmonocytes in microcytotoxicity test.We also explored the possible applica-tion of the monoclonal antibodies in VEC typing.