DETERMINATION OF GLYCOSAMINOGLYCEN IN URINE OF RATS FED WITH SYNTHETIC DIET CONTAINING DIFFERENT LEVEL OF SELENIUM / 西安交通大学学报(医学版)

ABSTRACT

The experiment was carried out on rats fed with synthetic diet containing torula yeasts with different levels of selenium, we collected their urine of 24 hours before the test on the 30th day and the 40th day of the test respectively. The level of acid glycosaminolycan in urine was determined by precipitate carbazole sulfate with zirconium oxychloride (ZrOCl_2) to measure glucuronate. The results by F test showed no significant difference in each group. The following two points have been reached. (a) The glucuronate level general rises in each group on the 30th day and declines on 40th day, especially in the low selenium group.(b) The more selenium the diet contains, the more glucuronate is excreted. The level of glucuronate in urine is correlated with that of blood selenium, with no significant difference.