STUDIES OF MONOCLONAL ANTIBODIES TO SYNGENIC MOUSE LEUKAEMIA ASCITIC CLONE CELLS / 中国免疫学杂志

ABSTRACT

4 mouse hybridoma cell lines stea-dily secreting monoclonal antibodiesagainst surfaced antigens of mouseleukaemia cells were obtained byfusing mouse SP2/0 myeloma cellswith SW-1 mouse spleen cells im-mnized with viable leukaemia asci-tic clone-1(LAC-1)cells,The McAbwas found especially to bind onlywith LAC-1 cells but not with nor-mal SW-1 mouse thymocytes andConA stimulated thymocytes orot her mouse tumor cells includingL_(6565),L_(783),SRS,L_(7712)mouseleukaemia as well as the leukaemiavirus isolated from LAC-1 cellculture supernatant by ELISA.The McAb lost its binding abilityafter absorption with LAC-1 cells.All of the McAbs were of IgG2b sub-classes and did not show any cytoto-xicity to LAC-1 cells in the presenceof complement in vitro,but theydid block the specific cytotoxicityto the targets affected by LAC-1cells induced Tc in ~(51)Cr releaseassay.