DETERMINATION ON GLUCURONATE OF THE MEDIA CULTURED CHONCHROCYTE WITE THE DIFFERENT CONCENTRATION OF SERUM FROM CALF AND HUMAN / 西安交通大学学报(医学版)

ABSTRACT

In this paper, We present the method of using glucuronate as a parameter to determine the growth of cartilage cell in the culture media. Using this method, we observed the metabolic condition of cartilage cell and knew the effect of the serum with the various types and different concentration to the growth of cartilage cell. The. experimental method was used for the first passage chondrocyte of rabbit. We put the cartilage cell in the culture media containing different concentration of serum from calf and human; then, observed the growth of cartilage cell under the microscope, collected the medium in given time; and determined the amount of glucuronate in the medium. Our results indicated that the first passage chondrocyte of rabbit can grow in the medium containing serum of calf and human in 5%～15% Also, the study shows that it is possible to determine the amount of glucuronate in the medium as a parameter to respond the growth of the cartilage cells.