Intracellular calcium ion in the activation of murine peritoneal macrophages for tumor cytolysis / 中国免疫学杂志

ABSTRACT

The importance of intracellular calcium ion in macrophage (M?) activation for tumor tcytolysis (MMTC) was investigated by using calcium channel blockers, nifedipine verapamil and intracellular calcium ion antagonists. nicardipine and ruthenium red. Nifedipine or verapamil did not suppress but significantly enhance M? activation by macrophageactivating factor (MAF) . However, nicardipine and ruthenium red bothinhibited M? activation by MAF and calcium ionophore A 23187. Activation of M? by lipopolysaccharide (LPS) was Likewise inhibited. These results suggest that extracellular catcium may not be involved in M? activation by MAF and M? activation by MAF, A23187, or LPS is intracellular calcium (?)on dependent.