Expression of human IL-2 receptor chain cDNA in vitro transcripts / 中国免疫学杂志

ABSTRACT

Recombinat plasmid pJSB13 has an insert of 121-937 bp of human IL-2R_? cDNA in multiple cloning site of pGEM3 vector.This plasmid was digested with EcoR I to linearize it and then in vitro transcripted with SP6 RNA polymerase and capped with cap structure analog 7mG (5') G.The capped transcripts were translated in rabbit reticutocyte lysate or Xenopus Iaevis oocytes respectively. The results demonstrated that they can be efficiently translated as functional mRNA in both systems. Moreover, the translation product, IL-2R_?,can be secreted into oocvte culture medium.The program we had used enables one to produce and identify mRNA and conse quently protein from any cDNA clone. Furthermore this research can be of much helpful to the researches on the relationship between structure, function and expression of IL-2R_?.