METHIONINE-ENKEPHALIN ALTERATION OF MITOGENIC AND MIXED LYMPHOCYTE CULTURE RESPONSES IN ZINC-DEFICIENT MICE / 西安交通大学学报(医学版)

ABSTRACT

The effects of methionine-enkephalin (M-EnK) on mitogenic and mixed lymphocyte culture (MLC) proliferation of splenocytes from Zn-deficient, restricted and control mice were evaluated. The data from this experiment showed that M-Enk could suppress the responses of splenocyte from th 3 groups to eoncanavalin A(Con A), but less inhibition was observed in the Zn-deficient group. M-Enk could also enhance the responses to Pokeweed Mitogen (PWM) and decrease the response to Lipopolysaceharide (LPS) in all groups. Alteration of proliferative responses to LPS,Con A and FWM was reversible in the presence of 10 ?mol/L naloxone, indicating that the effect of M-Enk on cellular proliferation was mediated by the opioid receptor. In the proliferation of MLC, the response of lymphocytes from Zndeficient mice was increased in the absence of M-Enk and M-Enk could suppress this increased response. It is therefore concluded that Met-Enk can modify the pattern of mitogenic responses and the alteration in Con A and MLC responses can be influenced by zinc deficiency.