A chemical method for preparing biotin-labeled nucleic acid probe / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)1986.
Article
in Chinese
| WPRIM
| ID: wpr-534952
ABSTRACT
A chemical method to prepare biotin-labeled nucleic acid probe for nonisotopic hybridization is introduced. The method involves the bisulfite-catalyzed transamination reaction of cytosine residues in nucleic acids with ethylenediamine or hexanediamine. Primary amino groups on the cytosine derivatives are then reacted with biotiny-N-hydroxysuccinimide ester. Results of dot blot and Southern blot carried out with biotinlabeled ? DNA probe show that such nonisotopic probe method is sensitive, specific, repeatable and unexpensive.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
1986
Type:
Article
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