STUDY ON THE EXPERIMENTAL CONDITIONS OF BMG-INDUCED CHONDROCYTE FORMATION IN VITRO / 西安交通大学学报(医学版)

ABSTRACT

Thigh skeletal muscle of 19d～21d(near-term) fetal rat cultured together with bone matrix gelatin (BMG) in vitro. The medium was DMEM containing 3700 mg/L NaCHO_3. After 1O d in culture, there were BMG-induced chondrocytes in 7 out of 8 explants. After 15、20 and 25 d in culture. BMG-induced chondrocytes were observed in all of the 30 expiants(10 explants in each point of time-period). The result shows that the experimental conditions and culture medium used in this paper were suitable for the system of BMG-induced choudrocyte formation in vitro.