CONSTRUCTION OF THE RECOMBINANT PLASMIDS CARYING BamHI C FRAGMENT OF HERPES SIMPLEX VIRUS I AND INSERTED THYMIDIN KINASE GENE-MINI Mu PHAGE DNA / 西安交通大学学报(医学版)

ABSTRACT

In order to mutate the BamHI C fragment of Herpes Simplex Virus 1 (HSV-1), the thymidin kinase gent-mini Mu phage DNA (TK-mM) was randomly inserted into the BamHI C fragment in the plasmid pRB 132 with DNA recombination technique. The six recombinant plasmids carrying the BamHI C fragment of HSV-1 and inserted TK-mM were isolated with DNA hybridization. The results of restriction endonucleasc analysis showed that the BamHI C fragment has been mutated by insertion of TK-mM system, and the insertional sites of TK-mM located in the six recombinant plasmids were different.