Stability of monoclonal antibody-secreting capacity of human-human hybridomas / 中国免疫学杂志

ABSTRACT

The stability of monoclonal antibody-secreting capacity of three human-h uman hybridomas was studied. It was noticed that there were some differences in the morphology, the size, the number of chromosomes and the secreting-capacity of human-human hybrid cells during expanding culture after cloning or for a long lime culture in vitro. The results showed that the stability of human monoclonal antibody-secreting was concerned with loss of some chromosomes or overgrowth of non-secreting population. The number of tetraploid cells was gradually increased after reselection using HAT medium to these different hybridomas. Meanwhile, some of cells which lost certain chromsomes died quickly. The capacity of monoclonal antibody secreting was recovered in some cell lines. It is important to clone the hybrid cells in time or reselect by using HAT medium when expanding the cloned cells.