MEASUREMENT OF HUMAN GROWTH HORMONE: DEVELOPMENT AND VALIDATION OF A SENSITIVE AND SPECIFIC ASSAY / 中华内分泌代谢杂志
Chinese Journal of Endocrinology and Metabolism
; (12)1985.
Article
in Zh
| WPRIM
| ID: wpr-535144
Responsible library:
WPRO
ABSTRACT
The determination of human growth hormor.e (hGH) in urine by means of LAB-ELISA technique was described. The major factors affecting the Sensitivity of LAB-ELISA were studied systematically. The optimized conditions have been established. The sensitivity of LAB-ELISA is at the level of pg/ml. There were little cross binding with human prolactin, thyroid-stimulating hormone, follicular-stimulating hormone, and luteinizing hormone. Human growth hormone levels in overnight urine in 50 normal children and 11 cases of GH-deficiency (GHD) were measurejl by the LAB-ELISA technique. Results of recovery test (recovery rate 100 ?1.4%) and precision test (intra-assay CV 4.72-12.00%, inter-assay CV 4.89-11.17%, and bias 0.20-12.00%)indicate that the method is highly specific,precise and stable.
Full text:
1
Index:
WPRIM
Type of study:
Diagnostic_studies
Language:
Zh
Journal:
Chinese Journal of Endocrinology and Metabolism
Year:
1985
Type:
Article