Preparation,Characterization and application of monoclonal antibodies to recombinant human interferon—?2a / 中国免疫学杂志

ABSTRACT

In this report,five mouse hybridoma cell lines secreting monoclonal antibodies to recombi-nant human interferon—?2a(rHu IFN—?2a)were raised.All the monoclonal antibodies with IgGl subclass react with rHu IFN—?2a detected by indirect ELISA with the titers ranging from 1320,000 to 110,240,000 but have no cross reactivity with rHu IFN?l,? and proteins from recombinant E.coli.Two of monoclonal antibodies,designated 3F1 and 5G10,have the ability to neutralize antiviral activity of rHu IFN—?2a.By a competitive ELISA the five monoclonal anti-bodies can be divided into three categories based on their epitope specificities.A sandwich ELISA established by employing 3F1 and 5G10 monoclonal antibodies could detect rHuIFN-?2a or rHuIFN-?2b as few as 60pg/ml?Moreover,3F1 monoclonal antibody displayed a satisfactoryproperty in affinity chromatography for rHuIFN?2a,which is able to match a monoclonal anti-body NK2 from CellTech Corporation.