A Novel Solid—phase Competitive TRFIA for Serum Progesterone / 中国免疫学杂志
Chinese Journal of Immunology
; (12)1985.
Article
in Zh
| WPRIM
| ID: wpr-535271
Responsible library:
WPRO
ABSTRACT
The acticle describes a time—resolved fluoroimmunoassay for serum progesterone,based ona competitive solid—phase antigen technique。Progesterone—BSA conjugates are coated onto thewhite opaque microtitration wells。Anti—progesterone antibody is biotinylated.Eu~(3+) are labelledto streptavidin as the tracer.The assay standard curve covers a range of 0.1—100ng/ml.Thelowest detecting limit is 0.05ng/ml。The intra and inter C?V s are 6.45% and 8.93% respec-tively.The average recovery is 89.5%.40woman samples are measured with RIA and presentTRFIA and the results are satisfactory,giving a correlation coefficient of 0.9619.The perfor-mance charicter—istics of the assay are discussed and compared with other known immunoas-says.
Full text:
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Immunology
Year:
1985
Type:
Article