Self—Made Fibrin Glue to treat Local Liver Trauma of Anticoagulated Rabbit / 中华创伤杂志

ABSTRACT

This experiment aims at exploring the prepared method of self—made fibrin glue (SMFG), its loeal hemostatic ways, curative effects and tissue reactions in the model of anticoagulated rabbits, to offer a experimental basis for clinical application. SMFG was made by a combination of swine thrombin and pyknotic fibrinogen extracted from fresh frozen plasma through double freezing, unfreezing, centrifugation—precipitation. Using SMFG in various liver wounds, local wounds were observed by gross, optical microscopic and electron microscopic ways. The results showed that SMFG had good hemostatic capacity in the model of heparinized liver trauma, without serious tissue reactions and cellular toxicity. It could satisfy the need of hemostasis in liver trauma, at the same time, it possesses some superiority of simple, safety and cheapness, and could be the best ideal alterative of commercial fibrin glue.