The obtaining of an anti-human CD40 mono-clonal antibody with special functions and the analysis of it's biological effects / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)1999.
Article
in Chinese
| WPRIM
| ID: wpr-535566
ABSTRACT
objective:
To prepare mouse anti-human CD40 antigen functional monoclonal antibody and to further study it's biological effects by triggering the Cd40 molecules on B cells and dendritic cells(DCs)Methods:
Using cell fusion, McAb screening immunofluorescence analysis immunoblotting and competition test obtain mouse anti-human CD40 McAb;by the proliferation assay of B cells and DCsand the analysis of expression of differentiation antigens on DCs.Results:
On the basis of phenotype analysis, Western blotting and competition test, it is verified that 5Cll recognizes human CD40 antigens specially; 5Cll can augement the proliferation of tonsil B cells in the LCD32cells and IL-4 culturing system; 5Cll can medicate DCs to get proliferation and maturatio.Conclusion:
5Cll+LCD32 +IL4 can make tonsil resting B cells survive and long-term proliferate in vitro, the setup of CD40 System furnish necessary tool for the study of B cells; McAb5Cll also triggered the generation, proliferation and maturation of dendritic cells from peripheral blood monocytes. Thus 5Cll is a McAb withspecial function and important application value.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
1999
Type:
Article
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