Antitumor effect of human MCP-1 on human osteosarcoma bearing nude mice / 中华骨科杂志
Chinese Journal of Orthopaedics
;
(12)2001.
Article
in Chinese
| WPRIM
| ID: wpr-535819
ABSTRACT
Objective To observe the inhibition effect of recombinant human monocyte chemoattractant protein- 1(MCP- 1) on implantation and growth of human osteosarcoma cells. Methods The method of protein fusion was used for the expression of MCP- 1 in b.coli and then the MCP- 1 was extracted and purified. Fifty nude mice were divided into 10 groups. For A1- 4 groups, the 5 mice in each group were locally injected with doses of 1? g, 10? g, 100? g, 1 mg of MCP- 1 at the same time when 4.4? 106 osteosarcoma cells were implanted in vivo. For B0- 4 groups, 5 mice in each group, the injection were given 2 weeks later when there was the formation of the tumor mass at doses of 0? g(0.2 ml normal saline), 1? g, 10? g, 100? g, 1 mg of MCP- 1 every other day. Five mice in group C were injected with dose of 0.2 ml NS as control. Results Implantation of osteosarcoma cells were completely prevented among mice of group A2- 4, tumor inhibiting effect even in group A1 and the rate of tumor inhibition was 69.69% . AKP values in mice of group A1- 4 were much lower than those of group B0(P
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Orthopaedics
Year:
2001
Type:
Article
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