Trophism of protein secreted by Schwann cell to motoneuron in culture / 中华显微外科杂志

ABSTRACT

Objective Exploration trophism of protein (relative molecular quantity 67?10 3) secreted by Schwann cell to motoneuron Methods Purfication of Schwann cell secretion neurotrophic protein (67?10 3) by method of Xiao Yuzhou Spinal cord ventral motoneuron (SCVMNs) of E 12～14 d (embryonic age 12～14 d) SD rats were harvested and cultured and identified SCVMNs were cultured in different concentration of protein (67?10 3) liquid Its neurobiological activity was assayed by MTT method Results The A values was not same in different concentration of protein (67?10 3) There was a dose effect relationship between biological effect intensity and protein dose, the optimum effect protein concentration of neurotrophic protein (67?10 3) was 20 ng/ml Conclusion The protein secreted by Schwann cell can maintain SCVMNs surval in culture