Comparison of the promoting effect on proliferation by secretory-and transmem-brane transforming growth factor-a / 中国免疫学杂志

ABSTRACT

Abstract Objective:The effects of both TM-TGFa and S-TGFa on proliferation were compared to explore the biological characteristics of the two forms of TGF-a.Methods:The proliferation of mouse fibroblast cell line NIH3T3 and its expression of PCNA were determined by the methods of MTT and histochemical technique. The level of IL-6 mRNA in HaCaT cell line was tested by in situ hybridization. Results:The two forms of TGFa were able to promote cell proliferation,PCNA expression and IL-6 mRNA transcription.The effects of S-TGFa had been shown stronger than that of TM-TGFa( P