Study on Effects of Estrogic Bisphenol_A on Vimentin in Sertoli Cells of Rats in Vitro / 环境与健康杂志

ABSTRACT

Objective To explore the mechanism of effect of estrogic bisphenol_A(BPA) on vimentin in Sertoli cells.Methods Immunohistochemical methods and situ hybridization technique were applied to analyze the effets of BPA on expressions of vimentin and protein kinase C(PKC) gene in primary cultured Sertoli cells.Results The results showed that BPA lengthened the Sertoli cells,the vimentin gene in Sertoli cells could be expressed completely in vitro,but BPA could prohibited translation of vimentin gene,however there was no direct relationship between BPA and vimentin protein degradation.PKC gene couldn't be expressed in Sertoli cells. Conclusion The results suggested that BPA prohibited translation of vimentin gene and therefore blocked the formation of cytoskeleton of Sertoli cells,and finally caused deformation of these cells,and that protein kinase C didn't disturb degradative metabolism of vimentin in Sertoli cells.