Effects of calcitonin gene-related peptide on calcification of human dental pulp cells in vitro / 实用口腔医学杂志
Journal of Practical Stomatology
;
(6)2001.
Article
in Chinese
| WPRIM
| ID: wpr-536502
ABSTRACT
砄bjective To investigate the effect of calcitonin gene related peptide(CGRP) on calcification function of human dental pulp cells(HDPCs) in vitro . Methods:
HDPCs cells were exposed to calcitonin gene related peptide at the dosages(mol/L) of 10 -12 , 10 -10 and 10 -8 for 3,6,9, 12 and 15 days respectively. Cell growth was studied by cell counting , alkaline phosphatase(AL) by a AL kit, osteocalin by immunoradiological tecnique and type I collagen mRNA expression by RT PCR.Results:
CGRP did not show any effect on the growth of HDPCs, but it increased the expression of AL, osteocalin and type I collagen mRNA in HDPCs in a dose dependent and not time dependent manner.Conclusion:
CGRP plays an important role in promoting differentiation and calcification function of HDPCs
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Practical Stomatology
Year:
2001
Type:
Article
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