Diagnostic utility of detection of CEA mRNA, CEA and CA19-9 in benign and malignant hydrothorax / 中国癌症杂志

ABSTRACT

Purpose:To determine the diagnostic value of detection of carcinoembryonic antigen mRNA (CEA mRNA), CEA and CA19-9 for diagnosis and differential diagnosis of malignant or benign hydrothorax. Methods:The groups included 76 cases of malignant hydrothorax group and 58 cases in benign hydrothorax group from whom specimens of chest fluid were obtined. CEA mRNA was detected by reverse transcriptase-nested primer-polymerase chain reaction (RT-NP-PCR). CEA and CA19-9 were detected by magnetic antibody immunoenzymetric assay (MAIA). Results:The positive rates of CEA mRNA, CEA and CA19-9 were 78.9%(60/76), 52.6%(40/76) and 55.3%(42/76) in the malignant hydrothorax group, and 8.6% (5/58), 5.2%(3/58) and 3.4%(2/58) in the benign dropsy of chest group respectively. There were significant differences in the positive results of CEA mRNA, CEA and CA19-9 between benign and malignant hydrothorax(P