The effect of synthetic osteogenic growth peptide on tibia fracture healing in rabbits / 中华骨科杂志

ABSTRACT

Objective To evaluate the effects of the synthetic osteogenic growth peptide (sOGP) on the healing of tibial fractures in rabbits. Methods Fifty two tibial fracture models were produced in the tibiae of New Zealand white rabbits and immobilized with a 2 mm diameter Kirschner wire as intramedullary nail and grouped by random. sOGP was administered to experimental animals by intravenous injection (0.5 ?g?kg-1 ?d-1) from the first day after surgery until the day before sacrifice, while the control animals were injected with saline solution. 3-5 rabbits from each group were killed at 1, 2, 3, 4, 6 weeks after fracture. The serum was obtained to determine the level of ALP and BGP by biochemistry and RIA respectively. The bone mineral density at fracture healing site was detected by DXA, the anteroposterior and lateral radiographs were taken, the histological examination was done and quantitative analysis of the callus area and the relative amounts of bone, cartilage, and fibrous tissue in the callus of each section were calculated by computer program. The 6 weeks tibial sample were tested biomechanically by universal material test machine. Results The serum ALP, BGP level were higher in sOGP injected groups than those in the controls. All tibia osteotomies healed uneventfully on radiograph. The area of the external callus was larger in the experimental groups than that in controls at 3,4 weeks after fracture; the average area was 265.44 mm2 at 3 weeks, 233.10 mm2 at 4 weeks in the experimental groups compared with that of 209.95 mm2 at 3 weeks and 209.21 mm2 at 4 weeks in the controls. The average bone mineral density at fracture healing site was slightly greater in the experimental group at 3,4,6 weeks, and the difference of the bone mineral density at 4 weeks has statistical significance (P