ELISA using Torpedo Acetylcholinesterase labelled second antibody / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)2001.
Article
in Chinese
| WPRIM
| ID: wpr-537099
ABSTRACT
Objective:
In order to build the method to apply Torpedo Acetylcholinesterase(AChE) labelled second antibody in ELBA.Methods:
The labelling method is NaIO4-mediated glycoprotein oxidation method.Results:
pH and temperature and the concentration of NaIO4 effect on labelling efficiency. The liter of AChE labelled IgG is higher than HPR labelled IgG.Conclusion:
Torpedo Acetylcholinesterase-labelled second antibody prepared could be applied and replaced the HRP-labelled second antibody in ELISA.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
2001
Type:
Article
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