Effects of laminin and collagen Ⅳ on the attachment and proliferation of Schwann cell / 中华显微外科杂志

ABSTRACT

Objective To detect the effects of laminin and type Ⅳ collagen on the attachment and proliferation of Schwann cells Method The plated were covered by laminin and collagen Ⅳ, the control groups were added antibody of laninin and collagen Ⅳ, collagen Ⅰ and polylysine The same concentration of Schwann cells were cultured in each groups plates The rates of cells attachment of each group were determined after 2,6,24 hours After 72 hours 3*"H TdR were mixed into culture matrix fluid and the cpm were mensurate Results The attachment rates of Laminin and collagen Ⅳ groups were higher than that of the control groups and the amount of mixed 3*"H TdR were also higher than that of control groups Conclusion Laminin and collagen Ⅳ promote the attachment and proliferation of Schwann cells